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Cellular response to <t>culture</t> in under <t>hypoxic</t> (1% O2) conditions. (A) HIF-1α expression in cells and (B) PlGF secretion from cells cultured in normoxia (21% O2) or hypoxia (1% O2) at 24, 48, and 72 hours. “ND” is not detectable. *p<0.05, ***p<0.001 and ****p<0.0001 by two-way ANOVA with post hoc Tukey. (C) HTR8, (D) JAR, and (E) BeWo growth curves during <t>cell</t> culture in normoxia and hypoxia over time. ***p<0.001 and ****p<0.0001 by Analysis of Covariance (ANCOVA). Additional statistical analyses are provided in Tables S8-S11.
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Cellular response to culture in under hypoxic (1% O2) conditions. (A) HIF-1α expression in cells and (B) PlGF secretion from cells cultured in normoxia (21% O2) or hypoxia (1% O2) at 24, 48, and 72 hours. “ND” is not detectable. *p<0.05, ***p<0.001 and ****p<0.0001 by two-way ANOVA with post hoc Tukey. (C) HTR8, (D) JAR, and (E) BeWo growth curves during cell culture in normoxia and hypoxia over time. ***p<0.001 and ****p<0.0001 by Analysis of Covariance (ANCOVA). Additional statistical analyses are provided in Tables S8-S11.

Journal: bioRxiv

Article Title: Investigating the Impact of Hypoxia and Syncytialization on Lipid Nanoparticle-Mediated mRNA Delivery to Placental Cells

doi: 10.1101/2025.06.23.661191

Figure Lengend Snippet: Cellular response to culture in under hypoxic (1% O2) conditions. (A) HIF-1α expression in cells and (B) PlGF secretion from cells cultured in normoxia (21% O2) or hypoxia (1% O2) at 24, 48, and 72 hours. “ND” is not detectable. *p<0.05, ***p<0.001 and ****p<0.0001 by two-way ANOVA with post hoc Tukey. (C) HTR8, (D) JAR, and (E) BeWo growth curves during cell culture in normoxia and hypoxia over time. ***p<0.001 and ****p<0.0001 by Analysis of Covariance (ANCOVA). Additional statistical analyses are provided in Tables S8-S11.

Article Snippet: For experiments using low oxygen cultures (termed “hypoxia” herein), a hypoxic cell culture chamber (StemCell Technologies) was purged with low oxygen gas (1% O2, 5% CO2, and 94% N2) for 5 minutes.

Techniques: Expressing, Cell Culture

The mechanisms of exosomal proteins in angiogenesis.

Journal: Biomedicines

Article Title: Exosomes in Skin Flap Survival: Unlocking Their Role in Angiogenesis and Tissue Regeneration

doi: 10.3390/biomedicines13020353

Figure Lengend Snippet: The mechanisms of exosomal proteins in angiogenesis.

Article Snippet: Hypoxic stem cells of apical papilla (SCAPs) , HIF-1α, Jagged-1 (JAG1) , HUVECs , Activates HIF-1α/Notch1 pathway, increases VEGF , Pro-angiogenesis , [ ] .

Techniques: Migration